Longitudinal study of the renin angiotensin aldosterone system in purebred Spanish broodmares during pregnancy

Thirty-one Purebred Spanish broodmares aged between 5 and 15 years old were studied for 11 months of pregnancy and compared to a control group composed of 11 non-pregnant Spanish mares. Morning venous blood samples were drawn on a monthly basis during pregnancy and pre-treated to prevent degradation until subsequent analysis. Serum REN, ANG-II and ALD concentrations were analysed by competitive immunoassay. This study found that pregnancy in Purebred Spanish broodmares is characterised by a gradual increase in REN concentrations, variable fluctuations in ALD concentrations, and no significant modifications in ANG-II concentrations. These results could provide potentially valuable information in understanding the physiological basis of the RAAS in mares, since we have been able to establish specific reference ranges for these components, as well as obtaining information on their evolution during pregnancy. As is often the case in other animal species, the increase in RAAS activity is a natural physiological process that occurs during pregnancy in Spanish broodmares. This may also be related to certain metabolic and hormone responses that contribute to the control of homeostasis in pregnant mares.