کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10893150 | 1082180 | 2014 | 22 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quality of chilled and cold-stored (5 °C) canine spermatozoa submitted to different rapid cooling rates
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Quality of chilled and cold-stored (5 °C) canine spermatozoa submitted to different rapid cooling rates Quality of chilled and cold-stored (5 °C) canine spermatozoa submitted to different rapid cooling rates](/preview/png/10893150.png)
چکیده انگلیسی
The aim of this study was to evaluate the sperm quality in chilled canine semen using diï¬erent cooling rates from room temperature (23 °C) to 5 °C and subsequently cold-stored at 5 °C for up to 96 hours. In experiment 1, semen samples from five dogs were pooled, diluted in Tris-fructose-citrate extender with 20% egg yolk and split into four aliquots that were chilled to 5 °C using diï¬erent cooling rates of 2.25, 0.9, 0.45, and 0.2 (control) °C/min. In experiment 2, semen from five dogs was processed individually as described above and split into two aliquots that were chilled to 5 °C using rates of either 2.25 °C/min or 0.2 °C/min. In both experiments, the sperm quality (i.e., sperm motility and viability) was evaluated before cooling and after 0, 24, 48, 72, and 96 hours of storage at 5 °C. The total motility, progressive motility, and quality of movement parameters were assessed using computer-assisted analysis system, and the percentage of viable spermatozoa was determined using ï¬ow cytometry (H-42/PI//FITC-PNA). The cooling rate did not inï¬uence the sperm quality parameters at any of the evaluation times. All evaluated males showed the same response to chilling semen at a rapid cooling rate. Storage time negatively inï¬uenced (P < 0.05) sperm motility, regardless of the cooling rate used. In conclusion, canine sperm could be chilled and stored for 96 hours at 5 °C in a Tris-fructose extender with 20% egg yolk using rapid cooling rates, with values for sperm quality similar to those from a conventional protocol.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 82, Issue 4, 1 September 2014, Pages 621-626
Journal: Theriogenology - Volume 82, Issue 4, 1 September 2014, Pages 621-626
نویسندگان
C. Rodenas, I. Parrilla, J. Roca, E.A. Martinez, X. Lucas,