کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10893302 | 1082195 | 2005 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Embryotropic effect of glycosaminoglycans and receptors in development of porcine pre-implantation embryos
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Embryotropic effect of glycosaminoglycans and receptors in development of porcine pre-implantation embryos Embryotropic effect of glycosaminoglycans and receptors in development of porcine pre-implantation embryos](/preview/png/10893302.png)
چکیده انگلیسی
The present study investigated the expression of receptors for glycosaminoglycans (GAGs) and the effect of GAGs supplementation on development of porcine IVF embryos. Total RNA was prepared from oocytes, 2-, 4- and 8-cell embryos, morulae and blastocysts. The expression of hyaluronic acid receptor (CD44) and heparin (HP) interacting protein (HIP) was determined using RT-PCR and Southern blot analysis. The CD44 and HIP mRNA were detected from in vitro matured oocytes and all stages of pre-implantation embryos. The IVF embryos were cultured in modified NCSU-23 medium supplemented with various concentrations (0, 0.1, 0.5 or 1.0Â mg/mL) of hyaluronic acid (HA) or heparin. Supplementing with 0.5Â mg/mL HA significantly increased total cell number compared to other experimental groups, due to increase in trophectoderm cells. Supplementing with 1.0Â mg/mL, HP significantly increased blastocyst formation rate compared to the control group. Supplementing media, in which IVF embryos were cultured with 0.5Â mg/mL HA + 1.0Â mg/mL HP, significantly increased blastocyst formation rate compared to the control group. In conclusion, the present study demonstrated the expression of HA and HP receptors and the embryotrophic effect of HA or HP on porcine IVF embryos.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 63, Issue 4, 1 March 2005, Pages 1167-1180
Journal: Theriogenology - Volume 63, Issue 4, 1 March 2005, Pages 1167-1180
نویسندگان
Hye-soo Kim, Gab-sang Lee, Sang-hwan Hyun, Dong-hyun Nam, So-hyun Lee, Yeon-woo Jeong, Sue Kim, Ji-hye Kim, Sung-keun Kang, Byeong-chun Lee, Woo-suk Hwang,