کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10899828 1084413 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
RRM1 maintains centrosomal integrity via CHK1 and CDK1 signaling during replication stress
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
RRM1 maintains centrosomal integrity via CHK1 and CDK1 signaling during replication stress
چکیده انگلیسی
DNA lesion-induced centrosomal abnormalities during the replication phase are relatively unknown. Here, we report that RNAi-mediated depletion of RRM1 induces cell-cycle arrest at the replication phase, along with severe DNA damage and centrosomal amplification. Interestingly, CHK1 depletion synergistically increased RRM1-depletion-induced centrosomal amplification. In response to hydroxyurea, CHK1 was delocalized from the centrosome by RRM1 depletion. Moreover, CDK1, which functions in centrosome separation and is inhibited by CHK1, was found to be essential for RRMI1-depletion-induced centrosomal amplification. Thus, we herein demonstrate that RRM1 preserves chromosomal stability via the CHK1- and CDK1-dependent stabilization of the centrosomal integrity at the replication stage.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 346, Issue 2, 1 May 2014, Pages 249-256
نویسندگان
, , , , , , , , ,