کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10899832 | 1084413 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of p53 increases chemosensitivity to 5-FU in nutrient-deprived hepatocarcinoma cells by suppressing autophagy
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
4′,6′-diamidino-2-phenylindole dihydrochloridepifithrin-αPFT-αGFP-LC3p53FITCGFP5FULC3DAPIHCC - HCCSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAAutophagy - اتوفاژیTem - این است5-fluorouracil - فلوروراسیل-۵، فلوئورواوراسیلNutrient deprivation - محرومیت از مواد مغذیTransmission electron microscopy - میکروسکوپ الکترونی عبوریHepatocarcinoma - هپاتوکارسینوماgreen fluorescent protein - پروتئین فلورسنت سبزmicrotubule associated protein 1 light chain 3 - پروتئین مرتبط با میکروتوبول 1 زنجیره سبک 3Propidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Activation of p53 can induce apoptosis, cell cycle arrest, and cell senescence, although some evidence has suggested that p53 could promote cell survival. However, whether p53 plays a positive role in cancer cell survival to chemotherapy remains unknown. In this study, we show that inhibition of p53 enhanced apoptosis and increased chemosensitivity to 5-fluorouracil (5-FU) in nutrient-deprived hepatocarcinoma cells (HCC). Meanwhile, nutrient-deprivation-induced autophagy was inhibited by pifithrin-α or small interfering RNA targeting p53. The expression of p53 was not increased when HCC were incubated under nutrient-deprived conditions. This indicates that the basal level of p53 is important to autophagy activation in nutrient-deprived HCC cells. Furthermore, combining p53 inhibition and nutrient deprivation or 5-FU treatment resulted in a marked increase in reactive oxygen species generation and mitochondrial damage. Antioxidants reduced nutrient deprivation or 5-FU-induced cell death of HCC after p53 inhibition. Our results suggest that p53 contributes to cell survival and chemoresistance in HCC under nutrient-deprived conditions by modulating autophagy activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 346, Issue 2, 1 May 2014, Pages 278-284
Journal: Cancer Letters - Volume 346, Issue 2, 1 May 2014, Pages 278-284
نویسندگان
Xian-ling Guo, Fei Hu, Shan-shan Zhang, Qiu-dong Zhao, Chen Zong, Fei Ye, Shi-wei Guo, Jian-wei Zhang, Rong Li, Meng-chao Wu, Li-xin Wei,