کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10899832 | 1084413 | 2014 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Inhibition of p53 increases chemosensitivity to 5-FU in nutrient-deprived hepatocarcinoma cells by suppressing autophagy
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
4′,6′-diamidino-2-phenylindole dihydrochloridepifithrin-αPFT-αGFP-LC3p53FITCGFP5FULC3DAPIHCC - HCCSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAAutophagy - اتوفاژیTem - این است5-fluorouracil - فلوروراسیل-۵، فلوئورواوراسیلNutrient deprivation - محرومیت از مواد مغذیTransmission electron microscopy - میکروسکوپ الکترونی عبوریHepatocarcinoma - هپاتوکارسینوماgreen fluorescent protein - پروتئین فلورسنت سبزmicrotubule associated protein 1 light chain 3 - پروتئین مرتبط با میکروتوبول 1 زنجیره سبک 3Propidium iodide - پروتئین یدید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Inhibition of p53 increases chemosensitivity to 5-FU in nutrient-deprived hepatocarcinoma cells by suppressing autophagy Inhibition of p53 increases chemosensitivity to 5-FU in nutrient-deprived hepatocarcinoma cells by suppressing autophagy](/preview/png/10899832.png)
چکیده انگلیسی
Activation of p53 can induce apoptosis, cell cycle arrest, and cell senescence, although some evidence has suggested that p53 could promote cell survival. However, whether p53 plays a positive role in cancer cell survival to chemotherapy remains unknown. In this study, we show that inhibition of p53 enhanced apoptosis and increased chemosensitivity to 5-fluorouracil (5-FU) in nutrient-deprived hepatocarcinoma cells (HCC). Meanwhile, nutrient-deprivation-induced autophagy was inhibited by pifithrin-α or small interfering RNA targeting p53. The expression of p53 was not increased when HCC were incubated under nutrient-deprived conditions. This indicates that the basal level of p53 is important to autophagy activation in nutrient-deprived HCC cells. Furthermore, combining p53 inhibition and nutrient deprivation or 5-FU treatment resulted in a marked increase in reactive oxygen species generation and mitochondrial damage. Antioxidants reduced nutrient deprivation or 5-FU-induced cell death of HCC after p53 inhibition. Our results suggest that p53 contributes to cell survival and chemoresistance in HCC under nutrient-deprived conditions by modulating autophagy activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 346, Issue 2, 1 May 2014, Pages 278-284
Journal: Cancer Letters - Volume 346, Issue 2, 1 May 2014, Pages 278-284
نویسندگان
Xian-ling Guo, Fei Hu, Shan-shan Zhang, Qiu-dong Zhao, Chen Zong, Fei Ye, Shi-wei Guo, Jian-wei Zhang, Rong Li, Meng-chao Wu, Li-xin Wei,