کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10900232 | 1084630 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
UV induced bystander signaling leading to apoptosis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
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چکیده انگلیسی
Human keratinocytes (HaCaT) were exposed to UV (A+B) (UVA-350-400Â mJ/cm2 and UVB-30Â mJ/cm2) which induces apoptosis as evidenced by MTT assay, DNA laddering, Bax and Fas up-regulation. UV induced apoptotic conditioned media (6Â h or earlier) did not cause apoptosis in unexposed cells. However, treatment with conditioned medium collected post UV exposure (1Â h) induced Bax in unexposed cells as observed by RT-PCR. The induction of cell death was initiated by conditioned medium collected 12Â h after UV exposure and the extent of death was increased progressively when conditioned medium collected 24 or 72Â h post UV exposure was used. Medium collected 24Â h after UV exposure also increased mitochondrial membrane permeability as determined by rhodamine uptake. Conditioned medium induced apoptosis did not involve reactive oxygen species (ROS) unlike UV induced apoptosis indicating that the apoptosis pathway could be different. Interestingly, at high dilution apototic conditioned medium did not induce apoptosis but actually protected cells from UV insult. The role of nerve growth factor (NGF) in UV induced bystander effects are also discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 223, Issue 2, 8 June 2005, Pages 275-284
Journal: Cancer Letters - Volume 223, Issue 2, 8 June 2005, Pages 275-284
نویسندگان
Gautam Banerjee, Nishma Gupta, Arun Kapoor, Govindarajan Raman,