کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10900459 | 1084646 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ellagic acid and quercetin interact synergistically with resveratrol in the induction of apoptosis and cause transient cell cycle arrest in human leukemia cells
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کلمات کلیدی
EQRG0/G1 phasePBSFACSFBSCQRDMSO - DMSOMTT - MTTApoptosis - خزان یاختهایfluorescence-activated cell sorting - دسته بندی سلول های فعال فلورسنسDimethylsulfoxide - دیمتیل سولفواکسیدCAM - ساخت به کمک کامپیوترCer - سرfetal bovine serum - سرم جنین گاوcombination index - شاخص ترکیبیAnti-cancer - ضدسرطانS phase - فاز SPhytochemicals - فیتوکمیکال هاPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریG2/M phase - مرحله G2 / MSynergy - همافزایی یا سینرژیPolyphenols - پلیفنلهاhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراControl - کنترل
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Anticarcinogenic effects of polyphenolic compounds in fruits and vegetables are well established. Although polyphenols naturally occur as combinations, little information is available regarding possible synergistic or antagonistic biochemical interactions between compounds. Identifying potential interactions between polyphenols may provide information regarding the efficiency of polyphenol-containing foods in cancer prevention. The objective of this study was to investigate the interactions of ellagic acid and quercetin with resveratrol, polyphenols which occur in muscadine grapes, with the hypothesis that the selected polyphenols would interact synergistically in the induction of apoptosis and reduction of cell growth in human leukemia cells (MOLT-4). To test this hypothesis, alterations in cell cycle kinetics, proliferation, and apoptosis (caspase-3 activity) were examined after incubation with ellagic acid, quercetin, and resveratrol as single compounds and in combination. Results showed a more than additive interaction for the combination of ellagic acid with resveratrol and furthermore, significant alterations in cell cycle kinetics induced by single compounds and combinations were observed. An isobolographic analysis was performed to assess the apparent synergistic interaction for the combinations of ellagic acid with resveratrol and quercetin with resveratrol in the induction of caspase 3 activity, confirming a synergistic interaction with a combination index of 0.64 for the combination of ellagic acid and resveratrol and 0.68 for quercetin and resveratrol. Results indicate that the anticarcinogenic potential of foods containing polyphenols may not be based on the effects of individual compounds, but may involve a synergistic enhancement of the anticancer effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 218, Issue 2, 10 February 2005, Pages 141-151
Journal: Cancer Letters - Volume 218, Issue 2, 10 February 2005, Pages 141-151
نویسندگان
Susanne U. Mertens-Talcott, Susan S. Percival,