کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10902456 1085046 2005 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthetic bile acid derivatives induce apoptosis through a c-Jun N-terminal kinase and NF-κB-dependent process in human cervical carcinoma cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Synthetic bile acid derivatives induce apoptosis through a c-Jun N-terminal kinase and NF-κB-dependent process in human cervical carcinoma cells
چکیده انگلیسی
Recently, we have reported that a synthetic derivative of ursodeoxycholic acid (UDCA), HS-1183, and those of chenodeoxycholic acid (CDCA), HS-1199 and HS-1200, induced apoptosis in human breast carcinoma cells through a p53-independent pathway. Here, we present that the synthetic bile acid derivatives induce apoptosis in SiHa human cervical carcinoma cells as well. The parental compounds, UDCA and CDCA, exhibited no significant effect on the cell viability at the concentration ranges tested. However, their synthetic bile acid derivatives significantly decreased cell viability in a concentration dependent manner. Characteristic manifestations of apoptosis including DNA fragmentation, an increased level of proapoptotic protein Bax, and cleavage of poly(ADP-ribose) polymerase were shown when the cells were treated with these synthetic compounds. Nuclear translocation of nuclear transcription factor NF-κB was increased and this suggests that the synthetic compounds induce apoptosis in a NF-κB dependent pathway. Phosphorylations of p38 and extracellular signal-regulated kinase were not affected, whereas c-Jun N-terminal kinase (JNK) was activated along with an increased level of transcription factor c-Jun. Our studies demonstrate that the newly synthesized bile acids are capable of inhibiting cell proliferation and inducing apoptosis in SiHa cells through activation of JNK and NF-κB.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 229, Issue 1, 8 November 2005, Pages 49-57
نویسندگان
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