کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10908758 1087803 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Alkylphosphocholines and curcumin induce programmed cell death in cutaneous T-cell lymphoma cell lines
ترجمه فارسی عنوان
آلکیل فسفوکولین ها و کورکومین موجب مرگ سلولی برنامه ریزی شده در سلول های سلولی لنفوم سلول های جلدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
چکیده انگلیسی
While most patients with early-stage cutaneous T-cell lymphomas (CTCL) have a very good prognosis, the survival of patients with extensive tumour stage and visceral involvement remains extremely poor and necessitates the development of more effective treatment modalities. In this study, we evaluated the in vitro effects of two alkylphosphocholines (APCs, miltefosine and erufosine) and the polyphenolic compound curcumin on 5 human CTCL cell lines (Hut-78, HH, MJ, My-La CD4+ and My-La CD8+). All tested drugs showed considerable cytotoxic activity, as determined by the MTT dye reduction assay. The IC50 values of both APCs ranged from the low micromolar level (Hut-78 cells) to 60-80 μM (HH cells). The IC50 values of curcumin ranged from 12 to 24 μM. All tested drugs induced apoptosis, as ascertained by morphological changes, DNA fragmentation and activation of caspase cascades. Miltefosine and erufosine induced dephosphorylation of Akt in My-La CD8+ cells and phosphorylation of JNK in Hut-78 and My-La CD8+ cells. APCs increased the level of the autophagic marker LC3B in Hut-78 and MJ cells. Results from co-treatment with autophagy modulators suggested that the cytotoxicity of APCs in CTCL cells is mediated, at least in part, by induction of autophagy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Leukemia Research - Volume 38, Issue 1, January 2014, Pages 49-56
نویسندگان
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