کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10914560 | 1088794 | 2016 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cripto: Expression, epigenetic regulation and potential diagnostic use in testicular germ cell tumors
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کلمات کلیدی
FACSYSTDCPGCCTeratoma - تراتوم، تراتوماELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاDiagnostic - تشخیصYolk sac tumor - تومور ساکشن ژلهdays post coitum - روز پس از مقاربتGerm cell cancer - سرطان سلول های زایمانTesticular germ cell cancer - سرطان سلول ژنتیکی مثبتSeminoma - سمینوماNon-seminoma - غیر سینموماfluorescence activated cell sorting - فلورسانس سلول فعال فعال سلولMethylation - متیلاسیونChoriocarcinoma - کریوکارسینوماCripto - کریپتو
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Type II germ cell tumors arise after puberty from a germ cell that was incorrectly programmed during fetal life. Failure of testicular germ cells to properly differentiate can lead to the formation of germ cell neoplasia in situ of the testis; this precursor cell invariably gives rise to germ cell cancer after puberty. The Nodal co-receptor Cripto is expressed transiently during normal germ cell development and is ectopically expressed in non-seminomas that arise from germ cell neoplasia in situ, suggesting that its aberrant expression may underlie germ cell dysregulation and hence germ cell cancer. Here we investigated methylation of the Cripto promoter in mouse germ cells and human germ cell cancer and correlated this with the level of CRIPTO protein expression. We found hypomethylation of the CRIPTO promoter in undifferentiated fetal germ cells, embryonal carcinoma and seminomas, but hypermethylation in differentiated fetal germ cells and the differentiated types of non-seminomas. CRIPTO protein was strongly expressed in germ cell neoplasia in situ along with embryonal carcinoma, yolk sac tumor and seminomas. Further, cleaved CRIPTO was detected in media from seminoma and embryonal carcinoma cell lines, suggesting that cleaved CRIPTO may provide diagnostic indication of germ cell cancer. Accordingly, CRIPTO was detectable in serum from 6/15 patients with embryonal carcinoma, 5/15 patients with seminoma, 4/5 patients with germ cell neoplasia in situ cells only and in 1/15 control patients. These findings suggest that CRIPTO expression may be a useful serological marker for diagnostic and/or prognostic purposes during germ cell cancer management.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular Oncology - Volume 10, Issue 4, April 2016, Pages 526-537
Journal: Molecular Oncology - Volume 10, Issue 4, April 2016, Pages 526-537
نویسندگان
Cassy M. Spiller, Ad J.M. Gillis, Guillaume Burnet, Hans Stoop, Peter Koopman, Josephine Bowles, Leendert H.J. Looijenga,