کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10915036 | 1089627 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DNA repair capacity measured by high throughput alkaline comet assays in EBV-transformed cell lines and peripheral blood cells from cancer patients and healthy volunteers
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We collected peripheral blood (PB) from 556 patients with various types of cancer who had undergone radiotherapy and from 81 healthy volunteers. We exposed whole PB and Epstein-Barr virus-transformed lymphoblastoid cell lines (EBLs) derived from the PB mononucleocytes to X-irradiation (5 Gy). Using the alkaline comet assay, we measured the immediate DNA damage and, at 15 min, the % residual damage. In PB, the immediate damage was similar in patients and healthy volunteers while the % residual damage (mean ± S.D.) was significantly higher in patients with breast (54.3 ± A23.9), cervical (54.7 ± A23.9), head/neck (56.8 ± A24.4), lung (60.1 ± 23.5), or esophageal cancers (59.5 ± A33.7) than in healthy donors (42.9 ± 19.6) (P < 0.05). We did not observe such differences in the EBV-transformed cell lines. Thus, radiation sensitivity of fresh PB cells measured by the alkaline comet assay was related to cancer status.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 588, Issue 1, 7 December 2005, Pages 1-6
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 588, Issue 1, 7 December 2005, Pages 1-6
نویسندگان
Mayumi Iwakawa, Miyako Goto, Shuhei Noda, Masashi Sagara, Shigeru Yamada, Naoto Yamamoto, Yoshihiro Kawakami, Yoshifumi Matsui, Yukimasa Miyazawa, Hideya Yamazaki, Hiroshi Tsuji, Tatsuya Ohno, Junetsu Mizoe, Hirohiko Tsujii, Takashi Imai,