Both RyRs and TPCs are required for NAADP-induced intracellular Ca2+ release

- Antibody against RyR1 reduced NAADP-evoked Ca2+ release by 81%.
- Combined inhibition of RyR1 and RyR3 (or RyR3-KO) reduced responses to NAADP by >90%.
- Knockout of TPC2 (or antibody against TPC2) reduced responses to NAADP by 64%.
- Combined inhibition of TPC2 and TPC1 reduced responses by 86%.
- In acidic stores inhibition of either pair of RyR1/3 or TPC1/2 abolished responses.