Evidence that NO/cGMP/PKG signalling cascade mediates endothelium dependent inhibition of IP3R mediated Ca2+ oscillations in myocytes and pericytes of ureteric microvascular network in situ

- Endothelium-dependent inhibition of Ca2+ oscillations in myocytes and pericytes was reversed by ODQ, an inhibitor of soluble guanylyl cyclase (sGC).
- Selective PKG inhibitor Rp-8-pCPT-cGMPS, reversed endothelium- dependent termination of agonist-induced Ca2+ oscillations in myocytes and pericytes.
- Selective PKG activator 8pCPT-cGMP induced inhibition of the agonist-induced Ca2+ oscillations in myocytes and pericytes.
- Inhibitory effect of SNAP was markedly enhanced by zaprinast.
- Inhibitory effect of NO/cGMP/PKG cascade is associated with suppressed Ca2+ release via IP3Rs of myocytes and pericytes.