Diversity of mitochondrial Ca2+ signaling in rat neonatal cardiomyocytes: Evidence from a genetically directed Ca2+ probe, mitycam-E31Q

- Development of genetically encoded mitochondrial Ca2+-probe with high Kd (1.6 μM).
- Simultaneous TIRF images of mitochondrial and cytosolic Ca2+ fluxes in cardiomyocytes.
- Big mitochondrial Ca2+ signals accompany SR Ca2+ release and transmembrane Ca2+ influx.
- Cellular Ca2+ overload accentuates rapid mitochondrial Ca2+ uptake and release.
- Identified 2 Ca2+-signaling populations of mitochondria: peripheral and perinuclear.