An algorithm for automated detection, localization and measurement of local calcium signals from camera-based imaging

- Local Ca2+ transients form the basis of cellular Ca2+ signaling.
- Here we describe a high-throughput algorithm for rapid detection/analysis of local Ca2+ signals.
- Noise filtering allows for the detection of small events in presence of fluctuating baselines.
- An intuitive user interface enables swift and precise evaluation of analyzed data.
- Amplitudes, kinetics, sub-pixel location and F/F0 ratio traces are output directly into Excel.