کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10926585 | 1091894 | 2005 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Calcium dynamics in catecholamine-containing secretory vesicles
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کلمات کلیدی
NAADPcytosolic [Ca2+]cyclic ADP riboseInsP3PC12VAMPcADPRFCCPeGFP[Ca2+]c - [Ca2 +] cnicotinic acid adenine dinucleotide phosphate - اسید نیکوتین اسید آدنین دینکلوتید فسفاتAequorin - اکوورینSecretory vesicles - واژگانی مخفیVesicle-associated membrane protein - پروتئین غشاء مرتبط با Vesicleenhanced green fluorescent protein - پروتئین فلورسنت سبز افزایش یافته استcatecholamine - کاتکولآمینهاcarbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone - کربونیل سیانید 4- (trifluoromethoxy) phenylhydrazoneCalcium - کلسیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Calcium dynamics in catecholamine-containing secretory vesicles Calcium dynamics in catecholamine-containing secretory vesicles](/preview/png/10926585.png)
چکیده انگلیسی
We have used an aequorin chimera targeted to the membrane of the secretory granules to monitor the free [Ca2+] inside them in neurosecretory PC12 cells. More than 95% of the probe was located in a compartment with an homogeneous [Ca2+] around 40 μM. Cell stimulation with either ATP, caffeine or high-K+ depolarization increased cytosolic [Ca2+] and decreased secretory granule [Ca2+] ([Ca2+]SG). Inositol-(1,4,5)-trisphosphate, cyclic ADP ribose and nicotinic acid adenine dinucleotide phosphate were all ineffective to release Ca2+ from the granules. Changes in cytosolic [Na+] (0-140 mM) or [Ca2+] (0-10 μM) did not modify either ([Ca2+]SG). Instead, [Ca2+]SG was highly sensitive to changes in the pH gradient between the cytosol and the granules. Both carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) and nigericin, as well as cytosolic acidification, reversibly decreased [Ca2+]SG, while cytosolic alcalinization reversibly increased [Ca2+]SG. These results are consistent with the operation of a H+/Ca2+ antiporter in the vesicular membrane. This antiporter could also mediate the effects of ATP, caffeine and high-K+ on [Ca2+]SG, because all of them induced a transient cytosolic acidification. The FCCP-induced decrease in [Ca2+]SG was reversible in 10-15 min even in the absence of cytosolic Ca2+ or ATP, suggesting that most of the calcium content of the vesicles is bound to a slowly exchanging Ca2+ buffer. This large store buffers [Ca2+]SG changes in the long-term but allows highly dynamic free [Ca2+]SG changes to occur in seconds or minutes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 37, Issue 6, June 2005, Pages 555-564
Journal: Cell Calcium - Volume 37, Issue 6, June 2005, Pages 555-564
نویسندگان
Alfredo Moreno, Carmen D. Lobatón, Jaime SantoDomingo, Laura Vay, Esther Hernández-SanMiguel, Rosario Rizzuto, Mayte Montero, Javier Alvarez,