کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10926588 | 1091894 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Altered Ca2+ sparks and gating properties of ryanodine receptors in aging cardiomyocytes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Altered Ca2+ sparks and gating properties of ryanodine receptors in aging cardiomyocytes Altered Ca2+ sparks and gating properties of ryanodine receptors in aging cardiomyocytes](/preview/png/10926588.png)
چکیده انگلیسی
To investigate the cellular mechanisms for altered cardiac function in senescence, we measured Ca2+ transients and Ca2+ sparks in ventricular cardiomyocytes from 6- to 24-month-old Fisher 344 (F344) rat hearts. The single channel properties of ryanodine receptors from adult and senescent hearts were also studied. In senescent myocytes, we observed a decreased peak [Ca2+]i amplitude and an increased time constant for decay (Ï), both of which correlated with a reduced Ca2+ content of the sarcoplasmic reticulum (SR). Our studies also revealed that senescent cardiomyocytes had an increased frequency of Ca2+ sparks and a slight but statistically significant decrease in average amplitude, full-width-at-half-maximum (FWHM) and full-duration-at-half-maximum (FDHM). Single channel recordings of ryanodine receptors (RyR2) demonstrated that in aging hearts, the open probability (Po) of RyR2 was increased but the mean open time was shorter, providing a molecular correlate for the increased frequency of Ca2+ sparks and decreased size of sparks, respectively. Thus, modifications of normal RyR2 gating properties may play a role in the altered Ca2+ homeostasis observed in senescent myocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 37, Issue 6, June 2005, Pages 583-591
Journal: Cell Calcium - Volume 37, Issue 6, June 2005, Pages 583-591
نویسندگان
Xinsheng Zhu, Beth A. Altschafl, Roger J. Hajjar, Héctor H. Valdivia, Ulrich Schmidt,