کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10926643 | 1091900 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ca2+ is mobilized by hydroxyl radical but not by superoxide in RTH-149 cells: The oxidative switching-on of Ca2+ signaling
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کلمات کلیدی
hypoxanthine/xanthine oxidaseRTHSarco-endoplasmic reticulum Ca2+-ATPasePMCAPLCU73122DHR4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl - 4-هیدروکسی 2،2،6،6-تترامتیلیکپیریدین-1-اکسیلROS - ROSCa2+-induced Ca2+ release - انتشار Ca2 + ناشی از Ca2 +dihydrorhodamine 123 - دی هیدرو رودامین 123SOD - سدTempol - سریعSuperoxide dismutase - سوکسوکس دیسموتازCa2+ signaling - سیگنالینگ Ca2 +Redox signaling - سیگنالینگ RedoxPlasma membrane Ca2+-ATPase - غشای پلاسما Ca2 + -ATPasephospholipase C - فسفولیپاز CSERCA - قلبFenton reaction - واکنش فنتونVerapamil - وراپامیلCICR - کمیته بینالمللی صلیب سرخReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Differential effects of superoxide and hydroxyl radical on intracellular calcium were investigated in trout hepatoma cells (RTH-149). [Ca2+]i variations were recorded using confocal imaging, fluo-3 loading, and exposure to various mixtures consisting of hypoxanthine/xanthine oxidase (HX/XO), and of sub-stimulatory concentrations of H2O2 and Cu2+. No [Ca2+]i variation was found with HX/XO, a slight [Ca2+]i rise with a mixture of Cu2+ and HX/XO, a sustained rise with Cu2+ and H2O2, and the highest rise with Cu2+, H2O2 and HX/XO. Fluorimetric assay using dihydrorhodamine 123 revealed a correlation between the oxidizing power of a mixture and its effect on [Ca2+]i. The [Ca2+]i rise induced by Cu2+, H2O2 and HX/XO, was partially reduced in Ca2+ free medium or in the presence of SOD, converted into Ca2+ transient by verapamil, and almost abolished by the PLC inhibitor U73122 or in the presence of the hydroxyl radical quencher TEMPOL. Data indicate that Ca2+ is mobilized by hydroxyl radical but not by superoxide. The mechanism consists of PLC activation causing intracellular Ca2+ release, while Ca2+ entry potentiates Ca2+ release thus leading to sustained [Ca2+]i rise. A role of hydroxyl radicals in the oxidative switching-on of Ca2+ signaling is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Calcium - Volume 38, Issue 5, November 2005, Pages 507-513
Journal: Cell Calcium - Volume 38, Issue 5, November 2005, Pages 507-513
نویسندگان
Bruno Burlando, Aldo Viarengo,