کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10927766 | 1092883 | 2015 | 29 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effect of ethanol induced mild stress on post-thawed bull sperm quality
ترجمه فارسی عنوان
اثر استرس خفیف ناشی از اتانول بر کیفیت اسپرم نیتروژن پس از انجماد
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کلمات کلیدی
بول، اسپرم، اتانول، یخ زدگی، خفیف
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
This study was performed to investigate the effect of sub-lethal exposure of bull semen to ethanol on the post-thaw spermatozoa quality. Semen samples (n = 24, 6 ejaculates/bull) from 4 Holstein bulls were collected and pooled. Pooled samples were divided into 4 equal parts and each part was frozen after being diluted with Optidyl® extender containing 0 (O-E0), 0.03 (O-E3), 0.09 (O-E9) and 0.15 (O-E15) % (v/v) absolute ethanol. Sperm motility and velocity, plasma membrane integrity and functionality, mitochondrial activity, malondialdehyde concentration, and apoptosis status were evaluated after thawing. A higher percentage of total motility was observed in the O-E9 group as compared to the O-E0, O-E3 and O-E15 groups (p < 0.05). Also, plasma membrane integrity was higher (p < 0.05) in the O-E9 group compared to the O-E3, and O-E15 groups. However, the difference between the O-E9 and O-E0 groups was not significant (p > 0.05). In terms of the proportion of sperm abnormality and plasma membrane functionality no differences (p > 0.05) were observed between the groups. Our results revealed that malondialdehyde level was lower in ethanol treated (O-E3, O-E9 and O-E15) groups compared to the O-E0 group (p < 0.05). Furthermore, the percentage of live spermatozoa with active mitochondria was higher in the O-E9 and O-E15 groups compared to the O-E0 and O-E3 groups (p < 0.05). The O-E3 and O-E9 groups resulted in the highest and lowest percentage of apoptotic spermatozoa, respectively (p < 0.05). The results of this study demonstrate that supplementation of semen extender with sub-lethal concentration of ethanol influences post-thawed bull sperm quality in a dose dependent manner.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 71, Issue 1, August 2015, Pages 12-17
Journal: Cryobiology - Volume 71, Issue 1, August 2015, Pages 12-17
نویسندگان
Hossein Vaseghi Dodaran, Mahdi Zhandi, Mohsen Sharafi, Elaheh Nejati-Amiri, Ardeshir Nejati-Javaremi, Abdollah Mohammadi-Sangcheshmeh, Mohamed Ahmed Mohamed Mahmoud Shehab-El-Deen, Malak Shakeri,