کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10928066 | 1092891 | 2013 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sperm quality and the morphology of cryopreserved testicular tissues recovered post-mortem from diverse wild species
ترجمه فارسی عنوان
کیفیت اسپرم و مورفولوژی بافت های بیضه منتقل شده پس از مرگ از گونه های مختلف وحشی
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کلمات کلیدی
بویید، سروده فیدل، انجماد تست،
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی
This study compared the effects of slow and fast freezing of testicular tissue of wild animals collected at post-mortem on testicular structure and testicular sperm. The testes of seven animals that had died in captivity; three felids (jungle cat, lion and leopard), two cervids (rusa deer and fea's muntjac) and two bovids (Sumatran serows) were cryopreserved using slow- and fast-freezing protocols. There were greater reductions in the integrity of the sperm membrane and DNA in tissues cryopreserved using slow freezing compared to fast freezing (membrane integrity reduced by 21.5 ± 12.4% vs. 13.0 ± 6.9%, P = 0.11 and DNA integrity reduced by 22.7 ± 16.3% vs. 6.6 ± 6.3%, P = 0.13). Histologically, there were similar degrees of detachment and shrinkage of the seminiferous tubules whereas, TUNEL assay revealed a tendency towards more apoptotic changes in the intra-tubular cells of tissues frozen using fast freezing compared to slow freezing (P = 0.09). In conclusion, fast freezing tended to cause less damage to testicular sperm but its protective effect on intra-tubular cells was likely compromised. This is the first report of gamete recovery in the wild and of the comparison in various wildlife species, between testicular tissues cryopreserved using different protocols.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 67, Issue 2, October 2013, Pages 244-247
Journal: Cryobiology - Volume 67, Issue 2, October 2013, Pages 244-247
نویسندگان
Paweena Thuwanut, Sayamon Srisuwatanasagul, Grisnarong Wongbandue, Nae Tanpradit, Ampika Thongpakdee, Daraka Tongthainan, Sukanya Manee-in, Kaywalee Chatdarong,