کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10928103 | 1092896 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries Development of a method for long-term preservation of Bombyx mori silkworm strains using frozen ovaries](/preview/png/10928103.png)
چکیده انگلیسی
Development of long-term preservation is essential for conservation of stocks of silkworm genetic resources. Thus far, a few methods have been reported, but more improvement is required for practical use. We have developed two effective modifications of a method for long-term preservation using frozen ovaries. One was slow cooling (1 °C per min) until â80 °C of the donor ovaries made possible by use of a BICELL freezing vessel. Using donor ovaries of 4th instar larvae, the average number of eggs laid per moth increased significantly from 110.7 ± 53.4 eggs per moth by slow cooling with the BICELL vessel vs 12.3 ± 10.3 eggs per moth by direct cooling in liquid nitrogen. A second improvement was connecting the thread bodies of the donor ovaries with those of the host in the transplantation step. Females operated on with the new method yielded a significantly higher percentage of moths that laid fertilized eggs than those transplanted with the standard procedure (70.4 ± 21.6% vs 22.9 ± 9.3%).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 66, Issue 3, June 2013, Pages 283-287
Journal: Cryobiology - Volume 66, Issue 3, June 2013, Pages 283-287
نویسندگان
Yutaka Banno, Kiyomi Nagasaki, Marino Tsukada, Yuko Minohara, Junko Banno, Kazuhiro Nishikawa, Kazunori Yamamoto, Kei Tamura, Tsuguru Fujii,