Cryopreservation-induced alterations in protein tyrosine phosphorylation of spermatozoa from different portions of the boar ejaculate

► We analyze sperm functions in two portions (P1 and SRF-P1) of the of boar ejaculate. ► During cryopreservation, the sperm velocity parameters differ between the portions. ► Protein tyrosine phosphorylation in spermatozoa do not differ between the portions. ► [Ca2+]i in spermatozoa also do not differ between the portions. ► A 32 kDa protein, not observed in fresh sperm, appeared during cryopreservation.