Development and subsequent cryotolerance of domestic cat embryos cultured in serum-free and serum-containing media

► We examined the serum effects on the development and cryotolerance of cat embryos. ► There was no serum effect on the embryonic development. ► Expanded blastocysts produced without serum exhibited better postthaw viability. ► The cryotolerance of cat embryos is influenced by the conditions of embryo culture.