Investigation of interleukin-1β release from cryopreserved blood stimulated with endotoxin

► Evaluate and standardize the cryopreservation of human blood for long term storage. ► Possibility of pooling of blood from multiple donors before cryopreservation. ► Assess the IL-1β release from cryopreserved blood in response to pyrogenic stimuli. ► Cryopreserved pooled blood is an experimentally viable alternative to fresh blood. ► Storage and supply of non-allergic, pathogen free blood for detection of IL-1β.