کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10928479 | 1092904 | 2012 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of cryoprotectant addition and washout methods on the viability of precision-cut liver slices
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کلمات کلیدی
CPAWmEPCLSMe2SOOsmosis - اسموزtissue banking - بانکداری بافتPrecision-cut liver slices - برش کبد دقیقDimethylsulfoxide - دیمتیل سولفواکسیدCryoprotectants - سرماخوردگیCryopreservation - سرماداری Cryoprotectant toxicity - سمیت کریو پروتئینVitrification - شیشهشدگیcryoprotective agent - عامل فریبنده حفاظتChemical toxicity - مسمومیت شیمیایی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم کشاورزی و بیولوژیک (عمومی)
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چکیده انگلیسی
With this method, CPA concentration versus time profiles were varied using various commercially available CPA mixtures [VMP, VM3, M22, and modified M22 (mM22)]. The viability of PCLS was determined after CPA loading and unloading and subsequent incubation during 3 h at 37 °C. Despite the reduction of osmotic stress, the viability of slices did not improve with gradual loading and unloading and remained considerably lower than that of untreated slices. The toxicity of the three CPA solutions did not correlate with either their potential osmotic effects or their total concentrations, and did not change strongly with exposure time in 100% CPA. The most likely explanation for these observations is that PCLS are not very sensitive to osmotic changes of the magnitude imposed in our study, and chemical toxicity of the CPA solutions is the main barrier to be overcome. The chemical toxicity of the CPAs used in this study probably originates from a source other than the total concentration of the solutions. The presented gradient method using the specially designed chamber is more time and cost effective than the step-wise method and can be universally applied to efficiently evaluate different CPA solutions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cryobiology - Volume 65, Issue 3, December 2012, Pages 179-187
Journal: Cryobiology - Volume 65, Issue 3, December 2012, Pages 179-187
نویسندگان
Na Guan, Sylvia A. Blomsma, Paul M. van Midwoud, Gregory M. Fahy, Geny M.M. Groothuis, Inge A.M. de Graaf,