Gene regulation by the lysine demethylase KDM4A in Drosophila

► KDM4A is a H3K36me3 demethylase in vivo. ► Gene regulation by Drosophila KDM4A does not always require demethylase activity. ► Many dKDM4A-regulated genes lack H3K36me3. ► Histone demethylation may not be required for regulation of some genes by dKDM4A. ► Over-expression of dKDM4 causes male lethality.