DCC mediated axon guidance of spinal interneurons is essential for normal locomotor central pattern generator function

► Mice lacking DCC show altered hindlimb coordination. ► DCC guides the neuronal components underlying left-right coordination circuitry. ► DCC is crucial for guidance of all spinal commissural subpopulations. ► DCC null mutants show a different locomotor phenotype than netrin deficient mice. ► V3 commissural interneurons control synchronous coordination over the midline.