کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10933714 | 1093823 | 2008 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Multiple roles of the furrow deepening Ca2+ transient during cytokinesis in zebrafish embryos
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
The generation of a required series of localized Ca2+ transients during cytokinesis in zebrafish embryos suggests that Ca2+ plays a necessary role in regulating this process. Here, we report that cortical actin remodeling, characterized by the reorganization of the contractile band and the formation during furrow deepening of pericleavage F-actin enrichments (PAEs), requires a localized increase in intracellular Ca2+, which is released from IP3-sensitive stores. We demonstrate that VAMP-2 vesicle fusion at the deepening furrow also requires Ca2+ released via IP3 receptors, as well as the presence of PAEs and the action of calpains. Finally, by expressing a dominant-negative form of the kinesin-like protein, kif23, we demonstrate that its recruitment to the furrow region is required for VAMP-2 vesicle transport; and via FRAP analysis, that kif23 localization is also Ca2+-dependent. Collectively, our data demonstrate that a localized increase in intracellular Ca2+ is involved in regulating several key events during furrow deepening and subsequent apposition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 316, Issue 2, 15 April 2008, Pages 228-248
Journal: Developmental Biology - Volume 316, Issue 2, 15 April 2008, Pages 228-248
نویسندگان
Wai Ming Li, Sarah E. Webb, Ching Man Chan, Andrew L. Miller,