کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10933894 | 1093870 | 2005 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A MAPK pathway is involved in the control of mitosis after fertilization of the sea urchin egg
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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![عکس صفحه اول مقاله: A MAPK pathway is involved in the control of mitosis after fertilization of the sea urchin egg A MAPK pathway is involved in the control of mitosis after fertilization of the sea urchin egg](/preview/png/10933894.png)
چکیده انگلیسی
Activation and role of mitogen-activated protein (MAP) kinase (MAPK) during mitosis are still matters of controversy in early embryos. We report here that an ERK-like protein is present and highly phosphorylated in unfertilized sea urchin eggs. This MAPK becomes dephosphorylated after fertilization and a small pool of it is transiently reactivated during mitosis. The phosphorylated ERK-like protein is localized to the nuclear region and then to the mitotic poles and the mitotic spindle. Treatment of eggs after fertilization with two different MEK inhibitors, PD 98059 and U0126, at low concentrations capable to selectively induce dephosphorylation of this ERK-like protein, or expression of a dominant-negative MEK1/2, perturbed mitotic progression. Our results suggest that an ERK-like cascade is part of a control mechanism that regulates mitotic spindle formation and the attachment of chromosomes to the spindle during the first mitosis of the sea urchin embryo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 282, Issue 1, 1 June 2005, Pages 192-206
Journal: Developmental Biology - Volume 282, Issue 1, 1 June 2005, Pages 192-206
نویسندگان
Wen Ling Zhang, Philippe Huitorel, Rainer Glass, Montserra Fernandez-Serra, Maria I. Arnone, Sandrine Chiri, André Picard, Brigitte Ciapa,