Functions of the mitotic B-type cyclins CLB1, CLB2, and CLB3 at mitotic exit antagonized by the CDC14 phosphatase

► The cdc14A280V allele has a mutation in the phosphatase domain. ► The cdc14A280V allele was employed to study CLB function at mitotic exit. ► Clb1 has the greatest effect on controlling cell cycle progression. ► Clb1 and Clb3 prevent the execution of cytokinesis. ► Clb2 exerts the greatest effect on actin ring formation.