کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10939706 | 1095321 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterisation and biosynthesis of d-erythroascorbic acid in Phycomyces blakesleeanus
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
d-Erythroascorbate and d-erythroascorbate glucoside have been identified in the Zygomycete fungus Phycomyces blakesleeanus. Ascomycete and Basidiomycete fungi also synthesise d-erythroascorbate instead of l-ascorbate, suggesting that d-erythroascorbate synthesis evolved in the common ancestor of the fungi. Both compounds accumulate in P. blakesleeanus at higher levels than observed in other fungal species. d-Erythroascorbate glucoside reduced dichlorophenolindophenol as effectively as l-ascorbate, but was more stable to autoxidation. d-Erythroascorbate glucoside predominated in spores and stationary phase mycelium. Free d-erythroascorbate accumulated during the exponential phase of mycelial growth and decreased to very low levels in the stationary phase. This suggests an association between growth and free d-erythroascorbate. P. blakesleeanus converted exogenous d-arabinose to d-erythroascorbate and its glucoside. A monomeric NAD-dependent d-arabinose dehydrogenase of 41Â kDa was purified to near homogeneity. The enzyme oxidised d-arabinose, l-galactose, and l-fucose. Correspondingly, mycelium converted exogenous l-galactose and l-fucose to l-ascorbate and 6-deoxyascorbate, respectively. The antioxidant role of d-erythroascorbate and its glucoside is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 42, Issue 5, May 2005, Pages 390-402
Journal: Fungal Genetics and Biology - Volume 42, Issue 5, May 2005, Pages 390-402
نویسندگان
Alberto Baroja-Mazo, Pilar del Valle, Javier Rúa, Sergio de Cima, Félix Busto, Dolores de Arriaga, Nicholas Smirnoff,