کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10940186 | 1095422 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Restricted expression of karyopherin alpha mRNA in the sea urchin suggests a role in neurogenesis
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کلمات کلیدی
nESKPNAIMPASWNPCNLSRanBP1LytechinusSAFRan binding protein 1artificial seawater - آب دریا مصنوعیSea urchin - توتیای دریایی، خارپُشت دریاییEmbryogenesis - جنین زاییNuclear transport - حمل و نقل هسته ایESC - خروجEmbryonic stem cell - سلول های بنیادی جنینیnuclear export signal - سیگنال صادرات هسته ایnuclear localization signal - سیگنال محلی سازی هسته ایnuclear pore complex - مجتمع مخرب هسته ایImportin - وارداتkaryopherin - کاییفرین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Karyopherin alpha (KAP-α) proteins are critical for the transport of many molecules into the nucleus. In this study, we identified three members of the KAP-α family in the sea urchin Lytechinus variegatus and described the developmental expression of these proteins. Although many importins are assumed to have ubiquitous expression, we found that all three genes were differentially expressed. Both LvKPNA1/5/6 and LvKPNA3/4 accumulated at high levels during cleavage, exhibiting cyclic expression as cells divided. By the blastula and gastrula stages expression decreased, remaining highest in the vegetal plate and archenteron, and by the prism/pluteus stages expression was restricted to the oral surface and gut. Expression of a third KAP-α gene, LvKPNA2/7, was examined in embryos from the mesenchyme blastula to pluteus stages. LvKPNA2/7 mRNA is present in vegetal cells of the mesenchyme blastula and, during gastrulation, it is localized to the archenteron and appears in additional groups of ectodermal cells. Prism/pluteus stage embryos expressed LvKPNA2/7 in the gut and scattered distribution of transcripts in the ciliary band resembled expression patterns of neural cells. We hypothesize that LvKPNA2/7 maintains pluripotency in the neural precursors prior to activation of neural differentiation and believe that this study is an important first step in an effort to better understand the roles of importins during embryogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Gene Expression Patterns - Volume 16, Issue 1, September 2014, Pages 51-60
Journal: Gene Expression Patterns - Volume 16, Issue 1, September 2014, Pages 51-60
نویسندگان
Christine A. Byrum, Jason Smith, Marietta R. Easterling, M. Catherine Bridges,