کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10940780 | 1095511 | 2016 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of the gene encoding component C3 of the complement system from the spider Loxosceles laeta venom glands: Phylogenetic implications
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کلمات کلیدی
ORFThioester-containing proteinsLoxoscelesTEDTEPCR2CR3CR1A2MAnaphylatoxin - آنافیلاواتکسینEST - استANA - اصلیphylogenetic analysis - تجزیه و تحلیل فیلوژنتیکExpressed Sequence Tag - تکرار اظهار نظرComplement C3 - تکمیل C3Maximum likelihood - حداکثر احتمالComplement system - دستگاه کمپلمان، سیستم کمپلمانMuscle - عضلهSPIDER - عنکبوتFactor B - فاکتور BFactor H - فاکتور Hopen reading frame - قاب خواندن بازMacroglobulin - ماکروگلوبولینAlpha-2 macroglobulin - ماگروگلوبولین آلفا 2multiple sequence comparison by log-expectation - مقایسه توالی چندگانه با انتظارات منطقیGene - ژن Complement receptor 3 - گیرنده تکمیلی 3complement receptor 1 - گیرنده مکمل 1complement receptor 2 - گیرنده مکمل 2
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A transcriptome analysis of the venom glands of the spider Loxosceles laeta, performed by our group, in a previous study (Fernandes-Pedrosa et al., 2008), revealed a transcript with a sequence similar to the human complement component C3. Here we present the analysis of this transcript. cDNA fragments encoding the C3 homologue (Lox-C3) were amplified from total RNA isolated from the venom glands of L. laeta by RACE-PCR. Lox-C3 is a 5178 bps cDNA sequence encoding a 190Â kDa protein, with a domain configuration similar to human C3. Multiple alignments of C3-like proteins revealed two processing sites, suggesting that Lox-C3 is composed of three chains. Furthermore, the amino acids consensus sequences for the thioester was found, in addition to putative sequences responsible for FB binding. The phylogenetic analysis showed that Lox-C3 belongs to the same group as two C3 isoforms from the spider Hasarius adansoni (Family Salcitidae), showing 53% homology with these. This is the first characterization of a Loxosceles cDNA sequence encoding a human C3 homologue, and this finding, together with our previous finding of the expression of a FB-like molecule, suggests that this spider species also has a complement system. This work will help to improve our understanding of the innate immune system in these spiders and the ancestral structure of C3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 221, Issue 9, September 2016, Pages 953-963
Journal: Immunobiology - Volume 221, Issue 9, September 2016, Pages 953-963
نویسندگان
D.T. Myamoto, G. Pidde-Queiroz, A. Pedroso, R.M. Gonçalves-de-Andrade, C.W. van den Berg, D.V. Tambourgi,