کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10940930 | 1095531 | 2014 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vitamin C treatment of mouse bone marrow-derived dendritic cells enhanced CD8+ memory T cell production capacity of these cells in vivo
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کلمات کلیدی
NF-κBPBSIFN-γCD8+ T cells - CD8 + سلول های Tinterleukin - اینترلوکینtumor necrosis factor alpha - تومور نکروز عامل آلفاDendritic cell - سلول دندریتیکDendritic cells - سلول های دندریتیکTNF-α - فاکتور نکروز توموری آلفاnuclear factor kappa-light-chain-enhancer of activated B cells - فاکتور هسته ای kappa-light-chain-enhancer از سلول های B فعال شده استPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریDendritic cell vaccination - واکسیناسیون سلول دندریتیکvitamin C - ویتامین سیInterferon gamma - گاما اینترفرون
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Vitamin C has been found to stimulate dendritic cells (DCs) to secrete more IL-12 and thereby drive naïve CD4+ T cells to differentiate into Th1 cells. In the present study, we evaluated the effect of these vitamin C-treated DCs on CD8+ T cell differentiation both in vitro and in vivo. Mouse bone marrow-derived DCs were prepared in the presence of GM-CSF and IL-15. With vitamin C treatment, these DCs, when LPS-stimulated, secreted more IL-12p70 and IL-15 than did untreated DCs. And when co-cultured with T cells, they yielded a higher frequency of IFN-γ+ CD8+ T cells. Moreover, we found that administering vitamin C-treated and tumor lysate-loaded DCs into mice yielded a higher frequency of CD44high CD62Llow CD8+ effector and effector memory T cells, which showed an increased ex vivo killing effect of the tumor cells. These DCs also elicited enhanced protective effects against inoculated tumor cells, most probably by way of the increased cytotoxic T cells, as was revealed by the decreased growth of the inoculated tumor cells in these mice. This ex vivo vitamin C treatment effect on DCs can be considered as a strategy for boosting DC vaccination potency against tumors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 219, Issue 7, July 2014, Pages 554-564
Journal: Immunobiology - Volume 219, Issue 7, July 2014, Pages 554-564
نویسندگان
Young-Joo Jeong, Jin-Hee Kim, Jun-Man Hong, Jae Seung Kang, Hang-Rae Kim, Wang Jae Lee, Young-il Hwang,