AMP-activated protein kinase connects cellular energy metabolism to KATP channel function

► During metabolic impairment, a change in the ATP:ADP ratio stimulates KATP channels opening. ► The ATP:AMP ratio is an acquisitively sensitive indicator of alterations in the metabolic status. ► We show that AMP-activated protein kinase (AMPK) activity promotes KATP channel opening. ► AMPK physically interacts with KATP channel subunits, suggestive of local signaling. ► Thus, small changes in AMP may trigger KATP channel availability under ischemic conditions.