A new approach to transcription factor screening for reprogramming of fibroblasts to cardiomyocyte-like cells

► Combinatorial screen of transcription factors for reprogramming to cardiomyocytes. ► Tbx5, Mef2c, Myocd was most efficient to induce Myh6, Myl2, Actc1, Nkx2.5, Scn5a. ► Tbx5, Mef2c, Gata4 lacked upregulation of Myl2. ► Reprogramming of NCFs resulted in functional cells having action potentials. ► In contrast, no function cells were obtained when reprogramming TTFs.