Embryonic expression of AMPK γ subunits and the identification of a novel γ2 transcript variant in adult heart

► We have identified a novel PRKAG2 transcript of intermediate length (γ2-3B). ► γ2-3B is the most abundant cardiac AMPK γ2 at both mRNA and protein levels. ► Functional changes in AMPK containing γ2-3B may mediate PRKAG2 cardiomyopathy. ► γ2 and γ3 are the early embryonic AMPK γ subuits.