کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10954067 | 1097834 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hic-5 deficiency enhances mechanosensitive apoptosis and modulates vascular remodeling
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کلمات کلیدی
DAPIterminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelingSMCEVGINF-γGAPDHPaxillinFAKECMLIM4′,6-diamidine-2′-phenylindole dihydrochloride - 4 '، 6-دیامیدین-2'-phenylindole dihydrochlorideROS - ROSMechanical stress - استرس مکانیکیTem - این استinterferon-γ - اینترفرون-γtumor necrosis factor-α - تومور نکروز عامل αTUNEL - تونلHic-5 - در اینجا 5Smooth muscle cell - سلول عضلانی صافsmooth muscle - عضله صاف TNF-α - فاکتور نکروز توموری آلفاExtracellular matrix - ماتریکس خارج سلولیTransmission electron microscope - میکروسکوپ الکترونی انتقالwild type - نوع وحشیHematoxylin and Eosin - هماتوکسیلین و ائوزینFocal adhesion - چسبندگی کانونیfocal adhesion kinase - کیناز چسبندگی کانونیglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Forces associated with blood flow are crucial not only for blood vessel development but also for regulation of vascular pathology. Although there have been many studies characterizing the responses to mechanical stimuli, molecular mechanisms linking biological responses to mechanical forces remain unclear. Hic-5 (hydrogen peroxide-inducible clone-5) is a focal adhesion adaptor protein proposed as a candidate for a mediator of mechanotransduction. In the present study, we generated Hic-5-deficient mice by targeted mutation. Mice lacking Hic-5 are viable and fertile, and show no obvious histological abnormalities including vasculature. However, after wire injury of the femoral artery in Hic-5 deficient mice, histological recovery of arterial media was delayed due to enhanced apoptosis of vascular wall cells, whereas neointima formation was enhanced. Stretch-induced apoptosis was enhanced in cultured vascular smooth muscle cells (vascular SMCs) from Hic-5 deficient mice. Mechanical stress also induced the alteration of intracellular distribution of vinculin from focal adhesions to the whole cytoplasm in SMCs. Immunoelectron microscopic study of vascular SMCs from a wire-injured artery demonstrated that vinculin was dispersed in the nucleus and the cytoplasm in Hic-5-deficient mice whereas vinculin was localized mainly in the sub-plasma membrane region in wild type mice. Our findings indicate that Hic-5 may serve as a key regulator in mechanosensitive vascular remodeling.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 50, Issue 1, January 2011, Pages 77-86
Journal: Journal of Molecular and Cellular Cardiology - Volume 50, Issue 1, January 2011, Pages 77-86
نویسندگان
Joo-ri Kim-Kaneyama, Naoki Takeda, Asami Sasai, Akira Miyazaki, Masataka Sata, Takahiro Hirabayashi, Motoko Shibanuma, Gen Yamada, Kiyoshi Nose,