کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10954314 | 1097894 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The role of p38 in the regulation of Na+-Ca2+ exchanger expression in adult cardiomyocytes
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: The role of p38 in the regulation of Na+-Ca2+ exchanger expression in adult cardiomyocytes The role of p38 in the regulation of Na+-Ca2+ exchanger expression in adult cardiomyocytes](/preview/png/10954314.png)
چکیده انگلیسی
The Na+-Ca2+ exchanger is crucial in the regulation of [Ca2+]i in the cardiac myocyte. The exchanger is upregulated in cardiac hypertrophy and failure. This upregulation can have an effect on calcium transients and possibly contribute to diastolic dysfunction and an increased risk of arrhythmias. Here we use adenovirus mediated gene expression to examine the role of p38 MAP kinase in upregulation of the exchanger in adult cardiac myocytes. We demonstrate that p38 mediates a part of the α-adrenergic stimulated upregulation of the Na+-Ca2+ exchanger gene. Overexpression of dominant-negative p38 isoforms and activated MKK3 and MKK6 in isolated adult cardiac myocytes demonstrates that p38 activation is sufficient for NCX1 promoter upregulation and that this is mediated primarily by the p38α isoform. Lastly, this work demonstrates that the p38α stimulated upregulation of the NCX1 promoter is mediated via the -80 CArG box element. This is the first time that a specific role for p38α in gene regulation has been demonstrated in isolated adult cardiomyocytes and provides an important clue to our understanding some of the factors regulating exchanger gene expression in the hypertrophic and failing heart.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 38, Issue 5, May 2005, Pages 735-743
Journal: Journal of Molecular and Cellular Cardiology - Volume 38, Issue 5, May 2005, Pages 735-743
نویسندگان
Lin Xu, Christiana S. Kappler, Donald R. Menick,