Transcription of the human EAP1 gene is regulated by upstream components of a puberty-controlling Tumor Suppressor Gene network

► Enhanced at Puberty 1 (EAP1) is an upper-echelon transcriptional regulator of puberty and reproductive function. ► Transcription of EAP1 mRNA is initiated at a preferred Transcription Start Site. ► The EAP1 promoter is activated by two transcription factors, TTF1 and CUX1-p200. ► The EAP1 promoter is repressed by EAP1 itself, YY1 and CUX1-p110. ► All four transcription factors are recruited in vivo to the EAP1 promoter.