کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10956547 | 1099384 | 2012 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neuropeptide Y and extracellular signal-regulated kinase mediate injury-induced neuroregeneration in mouse olfactory epithelium
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کلمات کلیدی
GBCPCNAMASH1mammalian achaete-scute homolog 1HBCPPADSOMPFGF2PFANPYERKPBS5-ethynyl-2′-deoxyuridine - 5-ethynyl-2'-deoxyuridineEdU - EDUMAPKs - MAPK هاProliferating Cell Nuclear Antigen - آنتیژن هسته ای تکثیر سلولیSynergistic effect - اثر سینرژیکolfactory epithelium - اپیتلیوم بویاییBrdU - بروموداکسی اوریدینbromodeoxyuridine - برومودسوویریدینCNS - دستگاه عصبی مرکزیOSN - سازمان ملل متحدcentral nervous system - سیستم عصبی مرکزیfibroblast growth factor 2 - عامل رشد فیبروبلاست 2olfactory sensory neuron - عصب حساس بویاییPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریparaformaldehyde - پارافرمالدهیدolfactory marker protein - پروتئین مارکر بویاییextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیmitogen-activated protein kinases - کیناز پروتئین فعال MitogenNeuropeptide Y - یوروپروتئین Y
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the olfactory epithelium (OE), injury induces ATP release, and subsequent activation of P2 purinergic receptors by ATP promotes neuroregeneration by increasing basal progenitor cell proliferation. The molecular mechanisms underlying ATP-induced increases in OE neuroregeneration have not been established. In the present study, the roles of neuroproliferative factors neuropeptide Y (NPY) and fibroblast growth factor 2 (FGF2), and p44/42 extracellular signal-regulated kinase (ERK) on ATP-mediated increases of neuroregeneration in the OE were investigated. ATP increased basal progenitor cell proliferation in the OE via activation of P2 purinergic receptors in vitro and in vivo as monitored by incorporation of 5â²-ethynyl-2â²-deoxyuridine, a thymidine analog, into DNA, and proliferating cell nuclear antigen (PCNA) protein levels. ATP induced p44/42 ERK activation in globose basal cells (GBCs) but not horizontal basal cells (HBCs). ATP differentially regulated p44/42 ERK over time in the OE both in vitro and in vivo with transient inhibition (5-15Â min) followed by activation (30Â min-1Â h) of p44/42 ERK. In addition, ATP indirectly activated p44/42 ERK in the OE via ATP-induced NPY release and subsequent activation of NPY Y1 receptors in the basal cells. There were no synergistic effects of ATP and NPY or FGF2 on OE neuroregeneration. These data clearly have implications for the pharmacological modulation of neuroregeneration in the olfactory epithelium.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Neuroscience - Volume 49, Issue 2, February 2012, Pages 158-170
Journal: Molecular and Cellular Neuroscience - Volume 49, Issue 2, February 2012, Pages 158-170
نویسندگان
Cuihong Jia, Colleen Cosgrove Hegg,