Acetyl-CoA metabolism in amprolium-evoked thiamine pyrophosphate deficits in cholinergic SN56 neuroblastoma cells

► Thiamine deficit decreases acetyl-CoA in cholinergic neuron mitochondria. ► Thiamine deficit causes depletion of acetyl-CoA in cholinergic neuron cytoplasm. ► Shortage of cytoplasmic acetyl-CoA suppresses acetylcholine synthesis. ► High expression cholinergic phenotype makes neurons susceptible to thiamine deficits. ► SN56 neuroblastoma cells may be cellular model thiamine-deficit encephalopathy.