Relationships Among Plasma Proteins, Hematological Values, and Gender in Thoroughbred and Miniature Horses

Our objective was to investigate the relationships among plasma proteins, hematological values, and gender in Thoroughbred (nine females and 16 males) and miniature (30 females and four males) horses. Twenty-two protein bands, ranging from 8 to 264 kDa, were detected in horse plasma. Red blood cells, hemoglobin, and hematocrit were negatively correlated (r ≤ −0.29; P < .05) with bands 13 and 17 but positively correlated (r ≥ 0.40; P < .01) with band 3. A negative correlation (r ≤ −0.27; P < .05) was detected between bands 1, 8, and lymphocytes. Neutrophils were positively correlated with bands 1 and 8. Basophils had a positive correlation (r = 0.36) with band 12 and negative correlation with bands 11 (r = −0.30) and 13 (r = −0.48). Platelets had positive correlations (r ≥ 0.24; P < .10) with bands 3, 4, 5, 10, 14, 19, and 22. The relative intensities of protein bands 4, 16, and 22 were affected (P < .05) by an interaction of breed and gender. Gender effects (P < .05) were detected for plasma protein bands 1 (1.6 vs. 2.4 ± 0.23), 5 (2.6 vs. 3.6 ± 0.25), and 11 (8.7 vs. 13.2 ± 1.1), respectively, for females and males. Breed effects (P < .05) were detected for plasma protein bands 5 (2.5 vs. 3.8 ± 0.25) and 11 (9.0 vs. 12.9 ± 1.1), respectively, for Thoroughbreds and miniatures. When developing new pathophysiological tools for equine disease diagnosis and therapy, breed and gender effects should be considered.