کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10962044 1102329 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assessment of potential causes of falsely positive Mycobacterium tuberculosis breath test
ترجمه فارسی عنوان
ارزیابی علل بالقوه تست نفس میکوکتریوم توبرکلوزیس منفی
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی میکروبیولوژی و بیوتکنولوژی کاربردی
چکیده انگلیسی
A suite of volatiles have previously been identified as specific markers of Mycobacterium tuberculosis metabolism in vitro. These markers - methyl phenylacetate, methyl p-anisate, methyl nicotinate, o-phenylanisole with the addition of methyl salicylate, may also be derived from other sources and confound development of a breath test for tuberculosis. To identify potential sources of these potential biomarkers food products, cosmetics, TB medication, environmental air and cigarette smoke were analysed for these markers using solid phase microextraction coupled with Gas Chromatography/Mass Spectrometry. Breath from healthy subjects, including smokers was also tested. Methyl salicylate was commonly detected, making this unsuitable as a specific marker for M. tuberculosis. Methyl nicotinate was detected repeatedly in cigarettes. Methyl phenylacetate was detected in 1.7% of healthy subjects and o-phenylanisole in just 1% of healthy breath indicating these may be more suitable for inclusion in the tuberculosis breath test due to their low “background” level. These results justify further clinical studies to further explore these markers as specific indicators of M. tuberculosis infection.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tuberculosis - Volume 93, Issue 3, May 2013, Pages 312-317
نویسندگان
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