کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971310 | 1106294 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of flea species using MALDI-TOF/MS
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
In the present study, a molecular proteomics (MALDI-TOF/MS) approach was used as a tool for identifying flea vectors. We measured the MS spectra from 38 flea specimens of 5 species including Ctenocephalides felis, Ctenocephalides canis, Archaeopsylla erinacei, Xenopsylla cheopis and Stenoponia tripectinata. A blind test performed with 24 specimens from species included in a library spectral database confirmed that MALDI-TOF/MS is an effective tool for discriminating flea species. Although fresh and 70% ethanol-conserved samples subjected to MALDI-TOF/MS in blind tests were correctly classified, only MS spectra of quality from fresh specimens were sufficient for accurate and significant identification. A cluster analysis highlighted that the MALDI Biotyper can be used for studying the phylogeny of fleas.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Immunology, Microbiology and Infectious Diseases - Volume 37, Issue 3, May 2014, Pages 153-157
Journal: Comparative Immunology, Microbiology and Infectious Diseases - Volume 37, Issue 3, May 2014, Pages 153-157
نویسندگان
Amina Yssouf, Cristina Socolovschi, Hamza Leulmi, Tahar Kernif, Idir Bitam, Gilles Audoly, Lionel Almeras, Didier Raoult, Philippe Parola,