کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971446 | 1106469 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional differentiation of spider hemocytes by light and transmission electron microscopy, and MALDI-MS-imaging
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
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چکیده انگلیسی
The most abundant cell types in the hemolymph of Cupiennius salei are plasmatocytes (70-80%) and granulocytes (20-30%). Both cells differ in shape, cytochemical and transmission electron microscopy staining of their cytoplasma and granules. According to MALDI-IMS (matrix-assisted laser desorption ionisation-mass spectrometry imaging), granulocytes exhibit ctenidin 1 (9510Â Da) and ctenidin 3 (9568Â Da), SIBD-1 (8675Â Da), and unknown peptides with masses of 2207 and 6239Â Da. Plasmatocytes exhibit mainly a mass of 6908Â Da. Unknown peptides with masses of 1546 and 1960Â Da were detected in plasmatocytes and granulocytes. Transmission electron microscopy confirms the presence of two compounds in one granule and cytochemical staining (light microscopy) tends to support this view. Two further hemocyte types (cyanocytes containing hemocyanin and prehemocytes as stem cells) are only rarely detected in the hemolymph. These four hemocyte types constitute the cellular part of the spider immune system and this is discussed in view of arachnid hemocyte evolution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 43, Issue 1, March 2014, Pages 59-67
Journal: Developmental & Comparative Immunology - Volume 43, Issue 1, March 2014, Pages 59-67
نویسندگان
Lucia Kuhn-Nentwig, Lukas S. Kopp, Wolfgang Nentwig, Beat Haenni, Kathrin Streitberger, Stefan Schürch, Johann Schaller,