کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971536 | 1106477 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular characterization of porcine Siglec-10 and analysis of its expression in blood and tissues
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular characterization of porcine Siglec-10 and analysis of its expression in blood and tissues Molecular characterization of porcine Siglec-10 and analysis of its expression in blood and tissues](/preview/png/10971536.png)
چکیده انگلیسی
Siglecs are sialic acid binding Ig-like proteins involved in the control of leukocyte responses. In this study we describe the characterization of a porcine orthologue of Siglec-10. A cDNA clone was obtained from a porcine library which encodes a protein with sequence homology to human Siglec-10. This cDNA codes for a type I transmembrane protein containing four Ig-like domains, a transmembrane region, and a cytoplasmic tail with three tyrosine-based motifs, including a membrane-proximal Grb2-binding motif, and two ITIM motifs. When expressed on transfected cells, porcine Siglec-10 was able to bind red blood cells in a sialic acid-dependent manner. Monoclonal antibodies were developed against this protein and used to examine its cell and tissue distribution in the pig. Siglec-10 was found to be expressed on blood B cells and B cell areas of the spleen and lymph nodes. A weak expression was also detected on monocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 48, Issue 1, January 2015, Pages 116-123
Journal: Developmental & Comparative Immunology - Volume 48, Issue 1, January 2015, Pages 116-123
نویسندگان
Z. Escalona, B. Álvarez, H. Uenishi, D. Toki, M. Yuste, C. Revilla, M. Gómez del Moral, F. Alonso, A. Ezquerra, J. DomÃnguez,