کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971596 | 1106483 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Genomic structure, characterization and expression analysis of a manganese superoxide dismutase from pearl oyster Pinctada fucata
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
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چکیده انگلیسی
Manganese superoxide dismutase (MnSOD) is a major component of the cellular defense mechanisms against oxidative damage. We cloned and analyzed the expression pattern and genomic structure of the MnSOD gene of pearl oyster Pinctada fucata, hereafter designated as PoMnSOD. The full-length PoMnSOD cDNA was 1080Â bp in length and consisted of a 5â²-untranslated region (UTR) of 222Â bp, a 3â²-UTR of 318Â bp with a polyadenylation signal (AATAAA) at 15 nucleotides upstream of the poly (A) tail, and an open reading frame (ORF) of 540Â bp encoding a polypeptide of 180 amino acids with an estimated molecular mass of 20.4Â kDa and a predicted pI of 6.72. Sequence analysis showed that PoMnSOD contained MnSOD family signatures F44NGGGHLNH52, I97QGSGWGWLA106 and D138VWEHAYY145, four conserved residues for manganese metal binding (H4, H52, D138 and H142), and two potential N-glycosylation sites (N33 and N51). Homology analysis revealed that PoMnSOD shared 47.6-55.9% identity and 57.4-65.6% similarity to the other known PoMnSOD amino acid sequences. PoMnSOD genomic DNA was 5040Â bp in length and contained three exons and two introns, which was a tripartite organization and coincided with the consensus GT-AG splicing rule. PoMnSOD promoter contained the various transcription factors associated with the immune modulation and stress responses. Quantitative RT-PCR analysis demonstrated that PoMnSOD was constitutively expressed in all detected tissues, and PoMnSOD mRNA expression was significantly up-regulated in intestine, mantle, gills, digestive gland and haemocytes after Vibrio alginolyticus injection. These results suggested that PoMoSOD was an acute-response protein involved in the innate immune responses of pearl oyster, and provided general information about the mechanisms of innate immune defense against bacterial infection in pearl oyster.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 41, Issue 4, December 2013, Pages 484-490
Journal: Developmental & Comparative Immunology - Volume 41, Issue 4, December 2013, Pages 484-490
نویسندگان
Dianchang Zhang, Shuge Cui, Huayang Guo, Shigui Jiang,