Molecular cloning and functional analysis of nucleotide-binding oligomerization domain 1 (NOD1) in olive flounder, Paralichthys olivaceus

► The gene encoding nucleotide-binding oligomerization domain 1 (NOD1) was cloned in olive flounder. ► NOD1 was expressed in all fish tissues examined. ► NOD1 mRNA levels was elevated in fish infected with Edwardsiella tarda, Streptococcus iniae, or VHSV. ► The inhibition of E. tarda and the increase of IL-1β levels were observed in NOD1 over-expressing cells.