کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10971673 | 1106504 | 2011 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular cloning, expression and antiviral activity of porcine interleukin-29 (poIL-29)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
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چکیده انگلیسی
Human interleukin-29 (IL-29) is a recently discovered cytokine displaying antiviral activity against a broad spectrum of viruses, designated as interferon (IFN)-λ1. We here report the molecular cloning, expression and antiviral activity of porcine IL-29 (poIL-29). The full-length poIL-29 cDNA sequence encoded 191 amino acids with a 19 amino acid signal peptide. Sequence alignments showed that poIL-29 had amino acid sequence similarity to wolf (76%), bear (75%), horse (75%), orangutan (73%), human (72%), cat (72%) and dog (70%) IL-29. The poIL-29 without signal anchor sequence was efficiently expressed as a 6ÃHIS fusion protein in Escherichia coli and the antiviral activity of the purified recombinant protein was 1.8 Ã 103 U/mg protein. The purified recombinant poIL-29 also exhibited significant antiviral effects against porcine reproductive and respiratory syndrome virus and pseudorabies virus in a dose-dependent manner, suggesting that poIL-29 is a potential antiviral agent against swine infectious diseases.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 35, Issue 3, March 2011, Pages 378-384
Journal: Developmental & Comparative Immunology - Volume 35, Issue 3, March 2011, Pages 378-384
نویسندگان
Dang Wang, Liurong Fang, Fuwei Zhao, Rui Luo, Huanchun Chen, Shaobo Xiao,